Long lived NMR signal in bone

Long lived NMR signal in bone.

Solids and rigid tissues, such as bone, ligaments, and tendons, typically appear dark in MRI, which is due to the extremely short-lived proton nuclear magnetic resonance signals. This short lifetime is due to strong dipolar interactions between immobilized proton spins, which render it challenging to detect these signals with sufficient resolution and sensitivity. Here we show the possibility o...

Drug Screening Boosted by Hyperpolarized Long-Lived States in NMR

Transverse and longitudinal relaxation times (T1ρ and T1) have been widely exploited in NMR to probe the binding of ligands and putative drugs to target proteins. We have shown recently that long-lived states (LLS) can be more sensitive to ligand binding. LLS can be excited if the ligand comprises at least two coupled spins. Herein we broaden the scope of ligand screening by LLS to arbitrary li...

Long-lived coherences for line-narrowing in high-field NMR.

a School of Chemistry, University of Southampton, Southampton SO17 1BJ, England b Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne, EPFL, Batochime, 1015 Lausanne, Switzerland Université Paris Descartes, UMR 8601 CNRS, 45, rue des Saints Pères 75006, Paris, France Université Pierre-et-Marie Curie, Paris, France Département de Chimie, Ecole Normale Supérieu...

Long-Lived Bone Marrow Plasma Cells Are

Long-lived states to monitor protein unfolding by proton NMR.

The relaxation of long-lived states (LLS) corresponds to the slow return to statistical thermal equilibrium between symmetric and antisymmetric proton spin states. This process is remarkably sensitive to the presence of external spins and can be used to obtain information about partial unfolding of proteins. We detected the appearance of a destabilized conformer of ubiquitin when urea is added ...